Fermentation extraction process
The extraction process of L-alanine by fermentation is shown in the figure below. The fermentation liquid is filtered to remove bacteria and macromolecular proteins, and then adjusted to a certain pH and flow rate through the 732 strong acid cation exchange resin (H+type). L-alanine is exchanged to the resin. Most of the pigments, soluble proteins and anions in the fermentation liquor are discharged with the overflow wastewater, and then the L-alanine on the resin is completely eluted with 4% ammonia water. The resin is decolorized by decompression, crystallization, centrifugation The finished L-alanine is obtained by drying and other steps. The refined mother liquor separated by centrifugation can be recycled to effectively improve the extraction yield.